In Vitro Transcription of Vesicular Stomatitis Virus: Initiation with GTP at a Specific Site Within the N Cistron

In vitro transcripts of vesicular stomatitis virus (VSV) were either 5′-terminally labeled by incorporation of [β-(32)P]GTP or were selected on Hg-agarose after incorporation of γ-thio-GTP. Capped RNAs ranged in size from 23 nucleotides, the shortest capped RNA detected, to full-length message size....

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Main Authors: Schubert, Manfred, Harmison, George G., Sprague, Judy, Condra, Cindra S., Lazzarini, Robert A.
Format: Artigo
Sprog:Inglês
Udgivet: 1982
Fag:
Animal Viruses
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC256107/
https://ncbi.nlm.nih.gov/pubmed/6286995
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