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Transfecting Human Neural Stem Cells with the Amaxa Nucleofector

Transfection of primary mammalian neural cells, such as human neural stem/precursor cells (hNSPCs), with commonly used cationic lipid transfection reagents has often resulted in poor cell viability and low transfection efficiency. Other mechanical methods of introducing a gene of interest, such as a...

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Bibliographic Details
Main Authors: Marchenko, Steven, Flanagan, Lisa
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2007
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2557122/
https://ncbi.nlm.nih.gov/pubmed/18997888
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/240
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