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Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
Plasmid DNA purification from E. coli is a core technique for molecular cloning. Small scale purification (miniprep) from less than 5 ml of bacterial culture is a quick way for clone verification or DNA isolation, followed by further enzymatic reactions (polymerase chain reaction and restriction enz...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
MyJove Corporation
2007
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2557117/ https://ncbi.nlm.nih.gov/pubmed/18997895 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/247 |
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