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Enzymatic Catalysis of Proton Transfer at Carbon: Activation of Triosephosphate Isomerase by Phosphite Dianion

More than 80% of the rate acceleration for enzymatic catalysis of the aldose-ketose isomerization of (R)-glyceraldehyde 3-phosphate (GAP) by triosephosphate isomerase (TIM) can be attributed to the 3′-phosphodianion group of GAP. We examine here the necessity of the covalent connection between the p...

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Autori principali: Amyes, Tina L., Richard, John P.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2007
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2556868/
https://ncbi.nlm.nih.gov/pubmed/17444661
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi700409b
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