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LOMA: A fast method to generate efficient tagged-random primers despite amplification bias of random PCR on pathogens

BACKGROUND: Pathogen detection using DNA microarrays has the potential to become a fast and comprehensive diagnostics tool. However, since pathogen detection chips currently utilize random primers rather than specific primers for the RT-PCR step, bias inherent in random PCR amplification becomes a s...

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Detalhes bibliográficos
Main Authors: Lee, Wah Heng, Wong, Christopher W, Leong, Wan Yee, Miller, Lance D, Sung, Wing Kin
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2553803/
https://ncbi.nlm.nih.gov/pubmed/18783594
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-9-368
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