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LOMA: A fast method to generate efficient tagged-random primers despite amplification bias of random PCR on pathogens
BACKGROUND: Pathogen detection using DNA microarrays has the potential to become a fast and comprehensive diagnostics tool. However, since pathogen detection chips currently utilize random primers rather than specific primers for the RT-PCR step, bias inherent in random PCR amplification becomes a s...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
BioMed Central
2008
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2553803/ https://ncbi.nlm.nih.gov/pubmed/18783594 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-9-368 |
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