A functional simian virus 40 origin of replication is required for the generation of a super T antigen with a molecular weight of 100,000 in transformed mouse cells.

We used two recombinant plasmids, one containing wild-type simian virus 40 DNA (pSVR1) and the other containing a simian virus 40 genome with a defective origin of replication (pSVR1-origin-minus) to transfect NIH3T3 cells. Quantitation of T-antigen synthesis by indirect immunofluorescence at 48 h a...

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Detalhes bibliográficos

Publicado no:	J Virol
Principais autores:	Chen, S, Grass, D S, Blanck, G, Hoganson, N, Manley, J L, Pollack, R E
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1983
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC255374/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6312105/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.48.2.492-502.1983
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