Using EGFP fusions to monitor the functional expression of GPCRs in the Drosophila Schneider 2 cells

In combining fluorescence measurements with ligand binding assays, the versatility of the EGFP C-terminally fused to the human mu opioid receptor (EGFP-hMOR) has been exploited to notably improve the expression level of functional G protein-coupled receptors in Drosophila S2 cells. A selected array...

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Bibliografiske detaljer
Main Authors: Brillet, Karl, Perret, Bénédicte G., Klein, Valérie, Pattus, Franc, Wagner, Renaud
Format: Artigo
Sprog:Inglês
Udgivet: Springer Netherlands 2008
Fag:
Special Issue: Heterologous Gene Expression
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2553644/
https://ncbi.nlm.nih.gov/pubmed/19003178
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-008-9125-4
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