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In-Depth Activation of Channelrhodopsin 2-Sensitized Excitable Cells with High Spatial Resolution Using Two-Photon Excitation with a Near-Infrared Laser Microbeam

We used two-photon excitation with a near-infrared (NIR) laser microbeam to investigate activation of channelrhodopsin 2 (ChR2) in excitable cells for the first time to our knowledge. By measuring the fluorescence intensity of the calcium (Ca) indicator dye, Ca orange, at different wavelengths as a...

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Detalhes bibliográficos
Main Authors: Mohanty, Samarendra K., Reinscheid, Rainer K., Liu, Xiaobin, Okamura, Naoe, Krasieva, Tatiana B., Berns, Michael W.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2553121/
https://ncbi.nlm.nih.gov/pubmed/18621808
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.108.130187
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