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Use of Protein Biotinylation In Vivo for Immunoelectron Microscopic Localization of a Specific Protein Isoform

Tagging of proteins in vivo by covalent attachment of a biotin moiety has emerged as a new prospective tool for protein detection and purification. Previously, we established a strategy for expression of in vivo biotinylated proteins in mammalian cells. It is based on coexpression of the protein of...

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Détails bibliographiques
Auteurs principaux: Viens, Antoine, Harper, Francis, Pichard, Evelyne, Comisso, Martine, Pierron, Gérard, Ogryzko, Vasily
Format: Artigo
Langue:Inglês
Publié: Histochemical Society 2008
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2544619/
https://ncbi.nlm.nih.gov/pubmed/18574249
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1369/jhc.2008.951624
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