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Hierarchical folding mechanism of apomyoglobin revealed by ultra-fast H/D exchange coupled with 2D NMR

The earliest steps in the folding of proteins are complete on an extremely rapid time scale that is difficult to access experimentally. We have used rapid-mixing quench-flow methods to extend the time resolution of folding studies on apomyoglobin and elucidate the structural and dynamic features of...

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Bibliografiske detaljer
Main Authors: Uzawa, Takanori, Nishimura, Chiaki, Akiyama, Shuji, Ishimori, Koichiro, Takahashi, Satoshi, Dyson, H. Jane, Wright, Peter E.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2008
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2544544/
https://ncbi.nlm.nih.gov/pubmed/18779573
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0804033105
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