Site-directed mutagenesis of proteinase 3C results in a poliovirus deficient in synthesis of viral RNA polymerase.

We used a synthetic double-stranded oligonucleotide to introduce amino acid substitutions into the proteinase 3C region of a poliovirus type 1 cDNA clone. The six different mutant viruses recovered exhibited a small-plaque phenotype when assayed on HeLa cells. Further investigation revealed that all...

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Detalles Bibliográficos

Publicado en:	J Virol
Autores principales:	Dewalt, P G, Semler, B L
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	American Society for Microbiology (ASM) 1987
Materias:	Research Article
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC254238/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3035216/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.61.7.2162-2170.1987
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