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Site-directed mutagenesis of proteinase 3C results in a poliovirus deficient in synthesis of viral RNA polymerase.

We used a synthetic double-stranded oligonucleotide to introduce amino acid substitutions into the proteinase 3C region of a poliovirus type 1 cDNA clone. The six different mutant viruses recovered exhibited a small-plaque phenotype when assayed on HeLa cells. Further investigation revealed that all...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Dewalt, P G, Semler, B L
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1987
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC254238/
https://ncbi.nlm.nih.gov/pubmed/3035216
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