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Numbers and Organization of RNA Polymerases, Nascent Transcripts, and Transcription Units in HeLa Nuclei

Using HeLa cells, we have developed methods to determine 1) the number of RNA polymerases that are active at any moment, 2) the number of transcription sites, and 3) the number of polymerases associated with one transcription unit. To count engaged polymerases, cells were encapsulated in agarose, pe...

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Detalhes bibliográficos
Main Authors: Jackson, Dean A., Iborra, Francisco J., Manders, Erik M.M., Cook, Peter R.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Cell Biology 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC25378/
https://ncbi.nlm.nih.gov/pubmed/9614191
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