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Detection and characterization of two ATP-dependent conformational changes in proteolytically inactive Escherichia coli Lon mutants by stopped flow kinetic techniques

Lon is an ATP dependent serine protease responsible for degrading denatured, oxidatively damaged and certain regulatory proteins in the cell. In this study we exploited the fluorescence properties of a dansylated peptide substrate (S4) and the intrinsic Trp residues in Lon to monitor peptide interac...

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Bibliografiske detaljer
Main Authors: Patterson-Ward, Jessica, Huang, Jon, Lee, Irene
Format: Artigo
Sprog:Inglês
Udgivet: 2007
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2537469/
https://ncbi.nlm.nih.gov/pubmed/17975895
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi701649b
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