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Detection and characterization of two ATP-dependent conformational changes in proteolytically inactive Escherichia coli Lon mutants by stopped flow kinetic techniques

Lon is an ATP dependent serine protease responsible for degrading denatured, oxidatively damaged and certain regulatory proteins in the cell. In this study we exploited the fluorescence properties of a dansylated peptide substrate (S4) and the intrinsic Trp residues in Lon to monitor peptide interac...

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Detalhes bibliográficos
Main Authors: Patterson-Ward, Jessica, Huang, Jon, Lee, Irene
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2537469/
https://ncbi.nlm.nih.gov/pubmed/17975895
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi701649b
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