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Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D 1. Mutagenesis and Structural Studies

Wild type green fluorescent protein (wt-GFP) and the variant S65T/H148D each exhibit two absorption bands, A and B, which are associated with the protonated and deprotonated chromophores respectively. Excitation of either band leads to green emission. In wt-GFP, excitation of band A (~390 nm) leads...

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Detalhes bibliográficos
Main Authors: Shu, Xiaokun, Kallio, Karen, Shi, Xinghua, Abbyad, Paul, Kanchanawong, Pakorn, Childs, William, Boxer, Steven G., Remington, S. James
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2536499/
https://ncbi.nlm.nih.gov/pubmed/17918959
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi7009037
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