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Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D 1. Mutagenesis and Structural Studies
Wild type green fluorescent protein (wt-GFP) and the variant S65T/H148D each exhibit two absorption bands, A and B, which are associated with the protonated and deprotonated chromophores respectively. Excitation of either band leads to green emission. In wt-GFP, excitation of band A (~390 nm) leads...
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Main Authors: | , , , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2007
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2536499/ https://ncbi.nlm.nih.gov/pubmed/17918959 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi7009037 |
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