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Infectious in vitro transcripts from cowpea chlorotic mottle virus cDNA clones and exchange of individual RNA components with brome mosaic virus.

Complete cDNA copies of genomic RNA1, RNA2, and RNA3 of cowpea chlorotic mottle virus (CCMV) were cloned 1 base downstream from a T7 RNA polymerase promoter. The mixture of capped in vitro transcripts from all three clones produced normal CCMV infections in barley protoplasts and cowpea plants. By u...

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Bibliographic Details
Published in:J Virol
Main Authors: Allison, R F, Janda, M, Ahlquist, P
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1988
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC253497/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3418781/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.62.10.3581-3588.1988
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