Establishment of a rat cell line inducible for the expression of human cytomegalovirus immediate-early gene products by protein synthesis inhibition.

Upon transfection of Rat-2-TK- cells with plasmid pES, containing the cloned 7.0-kilobase (kb) EcoRI-SalI fragment (0.063 to 0.089 map units) of the human cytomegalovirus genome, major immediate-early antigen expression was obtained in 1 to 2% of the nuclei of the transfected cells, as determined by...

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Detalhes bibliográficos

Publicado no:	J Virol
Principais autores:	Boom, R, Geelen, J L, Sol, C J, Raap, A K, Minnaar, R P, Klaver, B P, van der Noordaa, J
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1986
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC252992/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3009892/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.58.3.851-859.1986
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