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Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification
Linear RNA amplification using T7 RNA polymerase is useful in genome-wide analysis of gene expression using DNA microarrays, but exponential amplification using polymerase chain reaction (PCR) is still required for cDNA library preparation from single-cell quantities of RNA. We have designed a small...
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| 主要な著者: | , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
2008
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2528176/ https://ncbi.nlm.nih.gov/pubmed/18603591 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn420 |
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