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Toward an in vitro system for picornavirus assembly: purification of mengovirus 14S capsid precursor particles.

Mengovirus 14S subviral protein particles generated in infected L cells and in a cell-free translation system primed with mengovirus RNA were purified by sucrose gradient centrifugation and immunoaffinity chromatography. The preparations from both sources contained essentially pure proteins epsilon,...

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Detalles Bibliográficos
Publicado en:J Virol
Principais autores: Boege, U, Ko, D S, Scraba, D G
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 1986
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC252724/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3001350/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.57.1.275-284.1986
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