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Screening of Escherichia coli Temperature-sensitive Mutants by Pretreatment with Glucose Starvation

A system for screening Escherichia coli temperature-sensitive mutants is described. The system involves glucose starvation and minimizes ambiguities introduced by the interdependencies of macromolecular synthesis during balanced growth. The system permits the quick recognition of protein synthesis m...

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Bibliographic Details
Published in:J Bacteriol
Main Authors: Boyd, D., Nixon, R., Gillespie, S., Gillespie, D.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1968
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC252129/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4868351/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.95.3.1040-1050.1968
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