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Selection of Temperature-sensitive Activating Enzyme Mutants in Escherichia coli

A method based on temperature-conditional resistance to thymineless death has been designed to facilitate the isolation of amino acid-activating enzyme mutants. This method may be modified to obtain a greater or lesser proportion of activating enzyme mutants. In the latter instance, an increased pro...

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Bibliographic Details
Published in:J Bacteriol
Main Authors: Kaplan, Samuel, Anderson, Deborah
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1968
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Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC252122/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4868365/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.95.3.991-997.1968
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