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The Effect of the N-Terminus on Heme Cavity Structure, Ligand Equilibrium and Rate Constants and Reduction Potentials of Nitrophorin 2 from Rhodnius prolixus
The D1A mutant of recombinant NP2 has been prepared and shown to have the expression-initiation methionine-0 cleaved during expression in E. coli, as is the case for recombinant NP4, where Ala is the first amino acid for the recombinant protein, as well as for the mature native protein. The heme sub...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2518688/ https://ncbi.nlm.nih.gov/pubmed/17506528 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi7002263 |
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