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Determination of Kinetic Parameters for Interfacial Enzymatic Reactions on Self-Assembled Monolayers

This manuscript reports a method to characterize the kinetic constants for the action of enzymes on immobilized substrates. This example uses cutinase, a serine esterase that hydrolyzes 4-hydroxyphenyl valerate moieties that are immobilized on a self-assembled monolayer of alkanethiolates on gold. T...

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Bibliografiske detaljer
Main Authors: Nayak, Satish, Yeo, Woon-Seok, Mrksich, Milan
Format: Artigo
Sprog:Inglês
Udgivet: 2007
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2518328/
https://ncbi.nlm.nih.gov/pubmed/17402753
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/la062860k
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