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Determination of Kinetic Parameters for Interfacial Enzymatic Reactions on Self-Assembled Monolayers
This manuscript reports a method to characterize the kinetic constants for the action of enzymes on immobilized substrates. This example uses cutinase, a serine esterase that hydrolyzes 4-hydroxyphenyl valerate moieties that are immobilized on a self-assembled monolayer of alkanethiolates on gold. T...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2007
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2518328/ https://ncbi.nlm.nih.gov/pubmed/17402753 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/la062860k |
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