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Design and Implementation of Bimolecular Fluorescence Complementation (BiFC) Assays for the Visualization of Protein Interactions in Living Cells
Bimolecular fluorescence complementation (BiFC) analysis enables direct visualization of protein interactions in living cells. The BiFC assay is based on the discoveries that two non-fluorescent fragments of a fluorescent protein can associate to form a fluorescent complex and that the association o...
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2006
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2518326/ https://ncbi.nlm.nih.gov/pubmed/17406412 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2006.201 |
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