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The normalization of gene expression data in melanoma: Investigating the use of GAPDH and 18S rRNA as internal reference genes for quantitative real-time PCR
We examined a panel of 26 melanoma and fibroblast samples (tissues and cultured cells) to evaluate the suitability of two commonly used housekeeping genes, GAPDH and 18S rRNA, for quantitative real-time PCR. Both genes showed significant variations within the individual cell line and tissue groups....
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2515093/ https://ncbi.nlm.nih.gov/pubmed/18554498 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.05.024 |
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