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Purification of Sex Pili from Escherichia coli Carrying a Derepressed F-Like R Factor

A procedure for the purification of sex pili is described. Escherichia coli K-12 carrying Rldrd19 was grown in nutrient broth and blended at the time of peak sex pilus production. The cells were removed by centrifugation, and the supernatant fraction was concentrated, dialyzed, and clarified in an u...

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Autors principals: Beard, J. P., Howe, T. G. B., Richmond, M. H.
Format: Artigo
Idioma:Inglês
Publicat: 1972
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC251357/
https://ncbi.nlm.nih.gov/pubmed/4559831
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