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In vitro construction of poliovirus defective interfering particles.

To construct poliovirus defective interfering (DI) particles in vitro, we synthesized an RNA from a cloned poliovirus cDNA, pSM1(T7)1, which carried a deletion in the genome region corresponding to nucleotide positions 1663 to 2478 encoding viral capsid proteins, by using bacteriophage T7 RNA polyme...

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Main Authors: Hagino-Yamagishi, K, Nomoto, A
格式: Artigo
語言:Inglês
出版: 1989
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC251205/
https://ncbi.nlm.nih.gov/pubmed/2555563
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