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Zinc porphyrin: A fluorescent acceptor in studies of Zn-cytochrome c unfolding by fluorescence resonance energy transfer

FRET between the zinc porphyrin (ZnP) chromophore in zinc-substituted cytochrome c (Zn-cyt c) and an Alexa Fluor dye attached to specific surface sites was used to characterize Zn-cyt c unfolding. The use of ZnP as a fluorescent acceptor eliminates the need to doubly label the protein with exogenous...

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Autors principals: Ensign, Amy A., Jo, Iris, Yildirim, Ilyas, Krauss, Todd D., Bren, Kara L.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2008
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2504770/
https://ncbi.nlm.nih.gov/pubmed/18669660
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0802737105
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