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Separation of T-even Bacteriophage Deoxyribonucleic Acid from Host Deoxyribonucleic Acid by Hydroxyapatite Chromatography

A simple and rapid method is described for separation of T-even bacteriophage deoxyribonucleic acid (DNA) from host (Escherichia coli) DNA by hydroxyapatite column chromatography with a shallow gradient of phosphate buffer at neutral pH. By this method, bacteriophage T2, T4, and T6 DNA (but not T5,...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Autor principal: Oishi, M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1969
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC249910/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4889266/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.98.1.104-108.1969
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