Expression, purification, crystallization and preliminary X-ray analysis of Rv3117, a probable thiosulfate sulfurtransferase (CysA3) from Mycobacterium tuberculosis

The gene product of open reading frame Rv3117 from Mycobacterium tuberculosis (Mtb) strain H37Rv is annotated as encoding a probable rhodanese-like thiosulfate sulfurtransferase (MtbCysA3). MtbCysA3 was expressed and purified and then crystallized using the sitting-drop vapour-diffusion method. X-ra...

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Bibliografske podrobnosti
Main Authors: Witholt, Sarah J., Sankaranarayanan, Ramasamy, Garen, Craig R., Cherney, Maia M., Cherney, Leonid T., James, Michael N. G.
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2008
Teme:
Crystallization Communications
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2496863/
https://ncbi.nlm.nih.gov/pubmed/18540071
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108014449
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