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What is the role of the second “structural” NADP(+)-binding site in human glucose 6-phosphate dehydrogenase?

Human glucose 6-phosphate dehydrogenase, purified after overexpression in E. coli, was shown to contain one molecule/subunit of acid-extractable “structural” NADP(+) and no NADPH. This tightly bound NADP(+) was reduced by G6P, presumably following migration to the catalytic site. Gel-filtration yiel...

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Detalles Bibliográficos
Autores principales: Wang, Xiao-Tao, Chan, Ting Fai, Lam, Veronica M.S., Engel, Paul C.
Formato: Artigo
Lenguaje:Inglês
Publicado: Cold Spring Harbor Laboratory Press 2008
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2492815/
https://ncbi.nlm.nih.gov/pubmed/18493020
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.035352.108
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