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What is the role of the second “structural” NADP(+)-binding site in human glucose 6-phosphate dehydrogenase?
Human glucose 6-phosphate dehydrogenase, purified after overexpression in E. coli, was shown to contain one molecule/subunit of acid-extractable “structural” NADP(+) and no NADPH. This tightly bound NADP(+) was reduced by G6P, presumably following migration to the catalytic site. Gel-filtration yiel...
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| Auteurs principaux: | , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Cold Spring Harbor Laboratory Press
2008
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2492815/ https://ncbi.nlm.nih.gov/pubmed/18493020 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.035352.108 |
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