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Primase-based whole genome amplification

In vitro DNA amplification methods, such as polymerase chain reaction (PCR), rely on synthetic oligonucleotide primers for initiation of the reaction. In vivo, primers are synthesized on-template by DNA primase. The bacteriophage T7 gene 4 protein (gp4) has both primase and helicase activities. In t...

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Bibliografische gegevens
Hoofdauteurs: Li, Ying, Kim, Hyun-Jin, Zheng, Chunyang, Chow, Wing Huen A., Lim, Jeonghwa, Keenan, Brendan, Pan, Xiaojing, Lemieux, Bertrand, Kong, Huimin
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 2008
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2490742/
https://ncbi.nlm.nih.gov/pubmed/18559358
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn377
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