TROSY in triple-resonance experiments: New perspectives for sequential NMR assignment of large proteins

The NMR assignment of (13)C, (15)N-labeled proteins with the use of triple resonance experiments is limited to molecular weights below ∼25,000 Daltons, mainly because of low sensitivity due to rapid transverse nuclear spin relaxation during the evolution and recording periods. For experiments that e...

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Autores principales: Salzmann, Michael, Pervushin, Konstantin, Wider, Gerhard, Senn, Hans, Wüthrich, Kurt
Formato: Artigo
Lenguaje:Inglês
Publicado: The National Academy of Sciences 1998
Materias:
Biological Sciences
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC24862/
https://ncbi.nlm.nih.gov/pubmed/9811843
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