Development of a new protocol for 2-day generation of mature dendritic cells from human monocytes

We developed a new 2-day protocol for the generation of dendritic cells (DCs) from human monocytes in vitro. First, we demonstrated that 24 hours of culture with GM-CSF and IL-4 are sufficient to generate immature DCs capable of antigen uptake. We then compared two different strategies for DC matura...

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Détails bibliographiques
Auteurs principaux: Obermaier, Bianca, Dauer, Marc, Herten, Jan, Schad, Katharina, Endres, Stefan, Eigler, Andreas
Format: Artigo
Langue:Inglês
Publié: Biological Procedures Online 2003
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC248478/
https://ncbi.nlm.nih.gov/pubmed/14615816
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1251/bpo62
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