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Development of a new protocol for 2-day generation of mature dendritic cells from human monocytes
We developed a new 2-day protocol for the generation of dendritic cells (DCs) from human monocytes in vitro. First, we demonstrated that 24 hours of culture with GM-CSF and IL-4 are sufficient to generate immature DCs capable of antigen uptake. We then compared two different strategies for DC matura...
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| Main Authors: | , , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Biological Procedures Online
2003
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC248478/ https://ncbi.nlm.nih.gov/pubmed/14615816 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1251/bpo62 |
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