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Optical Lock-In Detection of FRET Using Synthetic and Genetically Encoded Optical Switches
The Förster resonance energy transfer (FRET) technique is widely used for studying protein interactions within live cells. The effectiveness and sensitivity of determining FRET, however, can be reduced by photobleaching, cross talk, autofluorescence, and unlabeled, endogenous proteins. We present a...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2480664/ https://ncbi.nlm.nih.gov/pubmed/18281383 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.124859 |
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