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Actin Polymerization Overshoots and ATP Hydrolysis as Assayed by Pyrene Fluorescence
We investigate via stochastic simulation the overshoots observed in the fluorescence intensity of pyrene-labeled actin during rapid polymerization. We show that previous assumptions about pyrene intensity that ignore the intensity differences between subunits in different ATP hydrolysis states are n...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
The Biophysical Society
2008
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2479571/ https://ncbi.nlm.nih.gov/pubmed/18390612 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.123125 |
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