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Linker insertion mutagenesis of herpesviruses: inactivation of single genes within the Us region of pseudorabies virus.
We describe a technique for the systematic inactivation of nonessential genes within the genome of a herpesvirus without the requirement for phenotypic selection. This technique is based on the insertion of an oligonucleotide containing translational stop codons at a random site within a large clone...
Gespeichert in:
| Veröffentlicht in: | J Virol |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology (ASM)
1990
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC247954/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2168958/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.64.10.4691-4696.1990 |
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