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Linker insertion mutagenesis of herpesviruses: inactivation of single genes within the Us region of pseudorabies virus.
We describe a technique for the systematic inactivation of nonessential genes within the genome of a herpesvirus without the requirement for phenotypic selection. This technique is based on the insertion of an oligonucleotide containing translational stop codons at a random site within a large clone...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC247954/ https://ncbi.nlm.nih.gov/pubmed/2168958 |
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