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Molecular cloning of the complete genome of strain GDVII of Theiler's virus and production of infectious transcripts.

We constructed a complete cDNA clone of strain GDVII of Theiler's virus in Bluescript plasmid. This recombinant plasmid, called pTMGDVII, was used to synthesize full-length RNA transcripts of the viral insert. This RNA was infectious for BHK cells. Virus R1-GDVII, obtained from transfected BHK cells...

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Détails bibliographiques
Publié dans:J Virol
Auteurs principaux: Tangy, F, McAllister, A, Brahic, M
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1989
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC247804/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2536823/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.63.3.1101-1106.1989
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