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Isolation of L-Forms of Bacillus subtilis Which Grow in Liquid Medium

L-forms of Bacillus subtilis can be isolated by treatment of the parent strain sequentially with N-methyl-N′-nitro-N-nitrosoguanidine and lysozyme and selection of the surviving protoplasts on semisolid medium containing 2,000 units of penicillin per ml. Some of these clones can be adapted to grow i...

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Detalles Bibliográficos
Main Authors: Young, Frank E., Haywood, Patricia, Pollock, Mary
Formato: Artigo
Idioma:Inglês
Publicado: 1970
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC247639/
https://ncbi.nlm.nih.gov/pubmed/4988045
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