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Minimally invasive determination of mRNA concentration in single living bacteria
Fluorescence correlation spectroscopy (FCS) has permitted the characterization of high concentrations of noncoding RNAs in a single living bacterium. Here, we extend the use of FCS to low concentrations of coding RNAs in single living cells. We genetically fuse a red fluorescent protein (RFP) gene a...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2475643/ https://ncbi.nlm.nih.gov/pubmed/18515347 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn329 |
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