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Template-directed addition of nucleosides to DNA by the BfiI restriction enzyme
Restriction endonucleases catalyse DNA cleavage at specific sites. The BfiI endonuclease cuts DNA to give staggered ends with 1-nt 3′-extensions. We show here that BfiI can also fill in the staggered ends: while cleaving DNA, it can add a 2′-deoxynucleoside to the reaction product to yield directly...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2475615/ https://ncbi.nlm.nih.gov/pubmed/18515343 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn343 |
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