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Comparison of Deoxyribonucleic Acid Uptake and Marker Integration in Bacilli and Protoplasts of Bacillus subtilis

trp(+)his(−) donor deoxyribonucleic acid (DNA) was added to highly competent trp(−)his(+) recipient bacilli and to protoplasts prepared from these bacilli, and the cell-DNA complexes were incubated for 30 min. The complexes were then washed and lysed, and their DNA was analyzed on a trp(−)his(−) str...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:J Bacteriol
Hauptverfasser: Miller, Irving L., Palmer, C. Dwayne, Landman, Otto E.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1972
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC247462/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4623311/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.110.2.661-666.1972
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