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Transformation of Bacillus subtilis: transforming ability of deoxyribonucleic acid in lysates of L-forms or protoplasts.

The transformation of Bacillus subtilis by homologous deoxyribonucleic acid (DNA) made available by gently lysing a stable L-form or protoplast suspension was 3 to 10-fold more efficient than DNA isolated by conventional procedures. This increased transformation was not influenced by digestion with...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Bettinger, G E, Young, F E
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1975
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC246150/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/807561/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.122.3.987-993.1975
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