The Combination of Chemical Fixation Procedures with High Pressure Freezing and Freeze Substitution Preserves Highly Labile Tissue Ultrastructure for Electron Tomography Applications

The emergence of electron tomography as a tool for three dimensional structure determination of cells and tissues has brought its own challenges for the preparation of thick sections. High pressure freezing in combination with freeze substitution provides the best method for obtaining the largest vo...

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Main Authors: Sosinsky, Gina E., Crum, John, Jones, Ying Z., Lanman, Jason, Smarr, Benjamin, Terada, Masako, Martone, Maryann E., Deerinck, Thomas J., Johnson, John E., Ellisman, Mark H.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2459253/
https://ncbi.nlm.nih.gov/pubmed/17962040
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2007.09.002
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