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Genetic Mapping in Bacillus subtilis by 5-Bromouracil Sensitization to Ultraviolet Inactivation of Transforming Activities

A new method for chromosome mapping of Bacillus subtilis Marburg is presented which is based on the sensitization to ultraviolet irradiation of transforming deoxyribonucleic acid that has incorporated 5-bromouracil instead of thymine. Deoxyribonucleic acid was extracted at intervals from the outgrow...

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Bibliographic Details
Published in:J Bacteriol
Main Authors: Matsumoto, Kouji, Shibata, Takehiko, Saito, Hiuga
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1974
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC245666/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4212031/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.119.3.666-671.1974
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