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DNA Detection and Signal Amplification via an Engineered Allosteric Enzyme
Rapid, sensitive, and sequence-specific DNA detection can be achieved in one step using an engineered intrasterically regulated enzyme. The semi-synthetic inhibitor-DNA-enzyme (IDE) construct (left) rests in the inactive state, but upon exposure to a complementary DNA sequence undergoes a DNA hybrid...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2453066/ https://ncbi.nlm.nih.gov/pubmed/12517141 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja027885u |
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