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Functional topography of nascent RNA in elongation intermediates of RNA polymerase

To determine the dynamics of transcript extrusion from Escherichia coli RNA polymerase (RNAP), we used degradation of the RNA by RNases T1 and A in a series of consecutive elongation complexes (ECs). In intact ECs, even extremely high doses of the RNases were unable to cut the RNA closer than 14–16...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Komissarova, Natalia, Kashlev, Mikhail
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 1998
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC24512/
https://ncbi.nlm.nih.gov/pubmed/9843952
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