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Functional topography of nascent RNA in elongation intermediates of RNA polymerase

To determine the dynamics of transcript extrusion from Escherichia coli RNA polymerase (RNAP), we used degradation of the RNA by RNases T1 and A in a series of consecutive elongation complexes (ECs). In intact ECs, even extremely high doses of the RNases were unable to cut the RNA closer than 14–16...

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Detalhes bibliográficos
Main Authors: Komissarova, Natalia, Kashlev, Mikhail
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC24512/
https://ncbi.nlm.nih.gov/pubmed/9843952
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