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Practical Method for Detection and Identification of Candida, Aspergillus, and Scedosporium spp. by Use of Rolling-Circle Amplification

A sensitive rolling-circle amplification (RCA)-based method utilizing species-specific padlock probes targeted to the internal transcribed spacer 2 region of the fungal ribosomal DNA gene complex was developed. The assay was rapid (2 hours) and specific. Of 28 fungal isolates (16 of Candida, six of...

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Main Authors: Zhou, Xiaoyong, Kong, Fanrong, Sorrell, Tania C., Wang, Hui, Duan, Yiqun, Chen, Sharon C. A.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 2008
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2446935/
https://ncbi.nlm.nih.gov/pubmed/18495860
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00420-08
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