Rapid and Sensitive Detection of Norovirus Genomes in Oysters by a Two-Step Isothermal Amplification Assay System Combining Nucleic Acid Sequence-Based Amplification and Reverse Transcription-Loop-Mediated Isothermal Amplification Assays

We developed a two-step isothermal amplification assay system, which achieved the detection of norovirus (NoV) genomes in oysters with a sensitivity similar to that of reverse transcription-seminested PCR. The time taken for the amplification of NoV genomes from RNA extracts was shortened to about 3...

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Detalhes bibliográficos
Main Authors: Fukuda, Shinji, Sasaki, Yukie, Seno, Masato
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2008
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2446545/
https://ncbi.nlm.nih.gov/pubmed/18456857
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00127-08
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