Expression, purification and characterization of a phyA(m)-phyCs fusion phytase

The phyA(m) gene encoding acid phytase and optimized neutral phytase phyCs gene were inserted into expression vector pPIC9K in correct orientation and transformed into Pichia pastoris in order to expand the pH profile of phytase and decrease the cost of production. The fusion phytase phyA(m)-phyCs g...

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Bibliografske podrobnosti
Main Authors: Zou, Li-kou, Wang, Hong-ning, Pan, Xin, Tian, Guo-bao, Xie, Zi-wen, Wu, Qi, Chen, Hui, Xie, Tao, Yang, Zhi-rong
Format: Artigo
Jezik:Inglês
Izdano: Zhejiang University Press 2008
Teme:
Biotechnology and Biochemistry
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2443349/
https://ncbi.nlm.nih.gov/pubmed/18600783
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1631/jzus.B0720006
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