A simple algorithm for quantifying DNA methylation levels on multiple independent CpG sites in bisulfite genomic sequencing electropherograms

DNA methylation at cytosines is a widely studied epigenetic modification. Methylation is commonly detected using bisulfite modification of DNA followed by PCR and additional techniques such as restriction digestion or sequencing. These additional techniques are either laborious, require specialized...

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Autors principals: Leakey, Tatiana I., Zielinski, Jerzy, Siegfried, Rachel N., Siegel, Eric R., Fan, Chun-Yang, Cooney, Craig A.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2008
Matèries:
Methods Online
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2441810/
https://ncbi.nlm.nih.gov/pubmed/18480118
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn210
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